Quality Education in the Benedictine Tradition
Mandy Raab, Ph.D., MSIS Director of Biotechnology Phone: 724-805-2705
This module focuses on Protein Synthesis, Purification, and Extraction. In a hands-on lab, students use microbiology techniques to culture transformed bacteria which contain a “mysterious” gene found in a particular plant from the rain forest. Through simple column chromatography, the students extract a Green-Fluorescent Protein (GFP) believed to be a new drug discovery. The isolated substance will glow in the dark. Students also learn about the process of translation through the use of Protein Synthesis Manipulatives as well as a paper-n-pencil exercise.