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Bruce D. Bethke, Ph.D.

Associate Professor of Biology and Director of Biotechnology

About Bruce Bethke

Dr. Bruce Bethke is a broadly trained biologist who has worked in various capacities with a wide variety of animal models and focused his graduate and two post-doctoral research projects on investigating genetic processes and developing systems to manipulate and deliver genes. Dr. Bethke's hobbies include fly fishing, fly tying, and beer brewing.


B.A. in Biology, The College of Wooster (1981)

M.S. in Molecular Genetics (1989), Ph.D. Molecular Genetics (1991), The Catholic University of America 


General Biology II

General Biology II Laboratory

Cell Biology

Cell Biology Laboratory

Molecular Genetics

Molecular Genetics Laboratory


Biotechnology Laboratory

Junior Research Seminar

Research Project

Research Thesis

Research Interests

My scientific interests are broad and consequently my research interest cover a wide variety of topics including both laboratory and field based studies. My expertise is in the areas of cell and molecular biology with a focus on genetic manipulation, gene expression, mammalian cell culture, and biotechnology.

Selected Publications

Bethke, B. D. and J. Golin. (1994) Long tract mitotic gene conversion in yeast: Evidence for a triparental contribution during spontaneous recombination. Genetics 137(2):439-453.

Bethke, B. D. and B Sauer (1997) Segmental genomic replacement by Cre-mediated recombination: genotoxic stress activation of the p53 promoter in single-copy transformants. Nucleic Acids Research 25 (14):2828-34.

Le, Y.; Gagneten, S.; Tombaccini, D.; Bethke, B. and B. Sauer. (1999) Nuclear targeting determinants of the phage P1 Cre DNA recombinase. Nucleic Acids Research 27 (24):4703-4709.

Bethke, B. D. and B. Sauer (2000) Rapid generation of isogenic mammalian cell lines expressing recombinant transgenes by use of Cre recombinase. Methods in Molecular Biology 133:75-84.

Soukharev, S.; Berlin, W.; Hanover, J. A.; Bethke, B. and B. Sauer. (2000) Organization of the mouse ASGR1 gene encoding the major subunit of the hepatic asialoglycoprotein receptor. Gene 241:233-240.